This study was to establish a molecular identification method to distinguish Dermacentor nuttalli(D. nuttalli) and Dermacentor marginatus(D. marginatus),and to study their phylogenetic relationship. The ticks were collected from domestic animals in Xinjiang Uygur Autonomous Region for morphological identification,then their genomic DNAs were isolated for amplifying mitochondrial 16 S rRNA and cytochrome oxidase subunit I gene(COI). The phylogenetic tree was constructed based on the sequencing results of PCR products by employing Mega 5. 0 and Mrbayes 3. 2 for homology analysis. Clustering analysis PCR product for 16 S rRNA from both D. marginatus and D. nuttalli was clustered together with their respective 16 S rRNA sequences previously accessed in GenBank,and that for COI gene as well. These are basically identical with morphological identification results. Our results indicate that morphological identification,combined with molecular markers,would be a simple and accurate method for distinguishing D. nuttalli and D. marginatus.
依据对虾黄头病毒（Yellow head virus,YHV）的非结构蛋白N基因序列,设计特异的锁式探针（Padlock probe,PLP）、检测探针及引物,建立YHV超分支滚环扩增（Hyper-branched rolling circle amplification,HRCA）检测试纸。灵敏度实验显示,YHV HRCA检测试纸能检测出的最低模板量为101拷贝,是RT-PCR灵敏度的100倍。特异性实验结果表明,该试纸能够特异性地对YHV进行检测。利用该检测试纸对进出口80批次虾样本进行检测,并将检测结果与常规RT-PCR相比较,结果显示,YHV HRCA检测试纸灵敏度方面优于常规RT-PCR方法,且操作简便、结果直观易读。
