Toll-like receptor(TLR)4 plays an important role in the innate immune system and has been involved in resistance/susceptibility to a number of diseases as revealed by studies in human and other domestic animals.Wild boar survives in natural environment without artificial interference and may be different from domestic pig in innate immune system.Here,the complete coding sequence of TLR4 and TLR4A was cloned in wild boar,and two other alternative splicing variants,TLR4B and TLR4C,were obtained.Compared to the counterpart from domestic pig(Gen Bank No.AJ628065),there were five SNPs,c.510T>C,c.960A>G,c.962A>G,c.1605T>G and c.1824A>G,in the coding sequence of wild boar TLR4A gene.TLR4 gene was expressed in all the tissues from wild boar studied with the most abundance in spleen tissue,and m RNA level was significantly lower in spleen from wild boar than that from Min pig.The allele distribution was significantly different at polymorphic loci c.962G>A and c.1027C>A(p<0.01)between wild boar and Min pig.The results would contribute to understand the innate immune system in wild boar.
Du XinZhuo Jian-shuJing Xiao-yanWang ChaoLiu Ying-ziYang Xiu-qin
原发性心肌症相关蛋白1(Cardiomyopathy-associated protein 1,CMYA1)基因是在横纹肌(骨骼肌)上特异表达的基因。猪CMYA1基因被认为是与背膘厚有关的QTL。本研究以猪CMYA1基因上存在的3个错义突变c.1394A>G(p.His465Arg)、c.1751A>G(p.Asp582Gly)和c.3290C>A(p.Thr1097Asp)为研究对象,利用PCR-RFLP方法分析其在大白、丹系长白、法系长白和北京黑猪4个群体中的分布情况。结果表明,在所检测的3个多态位点,各基因型在品种间的分布都存在显著差异(P<0.05);c.1394A>G和c.1751A>G都为中度多态位点(0.25G位点基因型在4个群体中的分布都符合Hardy-Weinberg平衡定律;序列分析表明c.1394A>G和c.1751A>G变异位点紧邻CMYA1蛋白的肌动蛋白结合域,并且c.1751A>G位点在人、猪、狗、牛、猕猴、小鼠等物种中高度保守。研究结果为进一步通过相关性分析揭示CMYA1基因变异与生长和胴体性状的相关性提供依据。
