In this paper, we introduce the concept of a g-Besselian frame in a Hilbert space and discuss the relations between a g-Besselian frame and a Besselian frame. We also give some characterizations of g-Besselian frames. In the end of this paper, we discuss the stability of g-Besselian frames. Our results show that the relations and the characterizations between a g-Besselian frame and a Besselian frame are different from the corresponding results of g-frames and frames.