[Objective] This study aimed to establish an AFLP reaction system for spinach. [Method] Modified CTAB method was used for DNA extraction from six varieties of spinach. Experimental conditions in enzyme digestion and ligation, pre-amplification and selective amplification in the AFLP reaction system were analyzed and optimized. [Result] The results showed that： （1） enzyme digestion had better effects in the PCR instrument than in a water bath and it was not sensitive to the digestion time and DNA concentrations; （2） a total volume of 20 μl, containing 1.2 μl of Mg2＋ （25 mmol/L）, 1.6 μl of dNTPs （2.5 mmol/L） and 0.2 μl of Taq-polymerase （5 U/μl） was the optimal condition for pre-amplification and selective amplification reactions. [Conclusion] The AFLP system established in this study was stable and efficient, which provided a theoretical basis for AFLP molecular marker analysis of genetic relationship between spinach varieties and genetic breeding.
[Objective] This study aimed to investigate the effect of hydrogen peroxide on the seed germination of eggplant （Solanum melongena L.） Luba 2.[Method] The seeds of Luba 2 were separately soaked in 20％,40％,60％ and 80％ hydrogen peroxide solution for 2,5 and 10 min before seed germination.The germination rate and days to reach the maximum germination rate were calculated.[Result] Treatment with low concentrations （20％） of hydrogen peroxide for 2 and 5 min made eggplant Luba 2 germinate in advance,and shortened the time to reach the maximum germination rate.Treatment with high concentrations （60％ and 80％） of hydrogen peroxide solution for longer time （10 min） caused significant damage to the seeds,delayed seed germination,and reduced the germination rate.[Conclusion] This study will provide theoretical references for the production practices of eggplant.
