NiO nanoparticles are prepared via thermal decomposition of nickel acetate coated by β-CD, and Characterized by using X-ray diffraction (XRD), transmission electron microscopy (TEM). Thermo-gravimetric (TG) analysis and electro spray mass spectrum (ESI-MS) are performed to investigate the thermal decomposition process and the possible formation mechanism of obtaining good morphology NiO nanoparticles by this method. Further, different CDs are used to prepare the precursors of inclusion complexes, and there are little affect of CD′s type on the NiO nanoparticles.
With the rapid development of bioanalytical methods and biosensors,it is desirable to immobilize a Self-as-sembled Monolayers with pure protein on inorganic surface.In this work,we tried to immobilize myoglobin on a surface of a silicon substrate as a model.At first,the cleaned silicon substrate was filled with5%3-aminopropy-ltrimethoxysilane(3-APTS).Then the formed SAMs were treated with glutaraldehyde and solution of myoglobin.Subsequently,the composition,structure and morphology of the thin film surface have been characterized by X-ray photoelectron spectroscopy(XPS)and Atomic force microscopy(AFM).The results indicate that myoglobin had been successfully immobilized on P100Si substrate.This method will be especially useful for further protein biomolecule immobilization and advanced single molecule detection studies.
