The tender stem of Rosa rugosa was used as explant and it suitable medium compositions were screened through uniform design experiments.The results showed that the tissue culture of Rosa rugosa required different kinds of culture medium in different phases.The most suitable culture media were as follows: B5+6-BA 2.90 mg·L-1 for shoots regeneration immediately at base of tender stem,the rate of regeneration was 96%;1/2 MS(macro-elemenyts)+IBA 0.07 mg·L-1 for rooting,the rooting rate was more than 98.5%.Plant regeneration system of Rosa rugosa had been successfully established.
The tender leafstalks of Ledum palustre var.dilatatum were used as explants for the experiment.Uniform design for the most suitable media for shoots regeneration immediately at base of tender leafstalks,rooting and germplasm conservation in vitro was screened.The results showed that N6+ZT 2.65 mg·L-1+IAA 0.05 mg·L-1 was fits for shoots regeneration,the frequency of shoots induction was higher than 92.5%;MS(modified)+IAA 0.1 mg·L-1+Kt 0.75 mg·L-1 for rooting,the rate of rooting was 98%;N-68+B9 2.5 mg·L-1+ Phloridzin 1.0 mg·L-1 for germplasm conservation in vitro for 46 months.Stems each with one node were cut from regenerated shoots and cultured for propagation,and a 90-fold proliferation rate was achieved within 30 days.The method of "deferring growth with dwarfing" was utilized for germplasm conservation in vitro at normal temperature.In vitro culture and germplasm conservation in vitro system of Ledum palustre var.dilatatum was established.
