’Pink Peace’rose was used to study the optimum conditions for transferring the SeNHX1 gene into the callus.The results showed that the optimal medium was MS+2,4-D 5.0 mg·L-1 + TDZ 0.5 mg·L-1.Agrobacterium tumefaciens-mediated transformation was able to take the target gene into callus and the blue spots were found.The optimum conditions for the transient expression of gusA gene are as following: bacterium density of OD was 0.5,infection time was 20 min,culture time was 3 days.Adding 100 μmol·L-1 AS,the frequency of transient expression of GUS gene was the highest,which reached about 85% in present study.
