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Cpne5 works as a negative factor in the neuronal migration: Cpne5 is a new Ca dependent phospholipids binding protein.Our Previous studies on Cpne5 showed that it was mai...; Xue-Feng DING,Yan WU,Yan-Rui WU,Fei WANG,Yong-Qi ZHAO,Ying WU,Shu-Hong LIU,Ling-Ling ZHU, Wen-Hong FAN~*,Ming FAN~* Department of Brain Protection & Plasticity Research,Beijing Institute of Basic Medical Sciences,Beijing 100850,China.; 关键词：NEURODEVELOPMENT; 文献传递

CPNE5的A结构域对肿瘤坏死因子α诱导核因子κB活化的影响被引量：1: 2008年; [目的]研究CPNE5的A结构域(AD)对肿瘤坏死因子α(TNF-α)激活核因子κB(NF-κB)转录活性的调控作用。[方法]用PCR法扩增AD序列,将其构建EGFP-N1载体上,在真核细胞中表达融合蛋白AD-GFP。以连有κB序列和表达水平受κB序列调控的荧光素酶基因的质粒为报告基因载体,将EGFP-N1、AD-GFP质粒分别与报告基因共转染到HEK293细胞中,经TNF-α处理后,用双荧光素酶报告基因系统测定荧光素酶的活性,检测它们对NF-κB转录活性的影响。[结果]构建了AD-GFP质粒;A结构域能够显著抑制NF-κB转录活性(P<0.05);AD对NF-κB转录活性的抑制具有剂量依赖性。[结论]CPNE5的A结构域能够剂量依赖性抑制NF-κB的转录激活活性。; 吴彦瑞王晓文靳雁斌吴海涛丁学峰刘淑红吴燕范文红范明; 关键词：肿瘤坏死因子

Cpne5,a new Ca2+ dependent phospholipids binding protein,works as a negative factor in the neuronal migration: Cpne5 is a new Ca dependent phospholipids binding protein.Our Previous studies on Cpne5 showed that it was mai...; Xuefeng DING,Yan WU,Fei WANG,Yongqi ZHAO,Ying WU,Shuhong LIU,Wenhong FAN,Lingling ZHU,Ming FAN Department of Brain Protection & Plasticity Research,Beijing Institute of Basic Medical Sciences,Beijing 100850, China; 关键词：NEURODEVELOPMENT; 文献传递

小鼠胎脑组织活体转基因技术的建立被引量：1: 2010年; 目的:建立实用的小鼠活体脑组织基因转染技术。方法:将EGFP质粒(CAGS启动子)注射到胎龄16 d(E16d)的胎鼠侧脑室,用镊形电极隔着子宫壁夹住胎鼠头部,在45 V电压下给予5次电脉冲刺激,每次刺激50ms,间隔1 s;转染后不同天数将胎鼠脑组织完整取出,以4%PFA固定后冰冻切片,进行激光共聚焦照相。结果:EGFP质粒被转入小鼠活体脑组织细胞中并获得表达,动物存活率为90%,GFP阳性率高于80%。结论:通过对麻醉剂、电脉冲刺激、质粒浓度、术中术后处理等多种实验条件的摸索,建立了实用的小鼠胎脑组织活体转基因技术。; 吴燕丁学锋刘淑红赵永岐吴彦瑞王飞范文红范明; 关键词：转基因神经元迁移胚胎

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国家自然科学基金(30871030)